SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. SpectraMax i3x readers. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Background subtraction was not necessary. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Download PDF. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Expand your lab’s capabilities to include flash applications such as dual luciferase and. 99 to $1999. user upgradeable. Automated for ease of use. detection cartridge LUM96: 59. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. As an aspirant Bioscience research with expertise in cell biology, immunology and bio instrumentation techniques, I bring a wealth of knowledge and expertise to the table. Find regional support. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. SoftMax Pro. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. HTRF IP standard curve. . Filterless readers provide consistent results with steady temperature regulation. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Average 98 stars, based on 1 article reviews. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Unlimited breadth of. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Rutin was used to generate standard curves for quantification. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The CellTiter-Glo assay from Promega uses luciferase. . 313 reference gain check fail. The assay can accurately test the toxicology of compounds as shown in Figure 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. Ease of use. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Version 7. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 303 unable to cal dark current. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Examples: “Press carefully to engage,” “ Do not press down. SpectraMax i3x and Paradigm readers require the FP-FLUO. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Description 1. BMG LABTECH VANTAstar. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Recommended settings for SpectraMax iD5 and i3x readers. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Figure 2-23: Bottle holder To install the bottle holder: 1. Additionally, user-installable options allow the SpectraMax i3 System to. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The plate was shaken and incubated in the dark for 10 minutes. Failed to load PDF file. *On the SpectraMax i3x reader only. com. Summary of Contents for Molecular Devices SpectraMax M2. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Register to download your free eBook today. . Average 98 stars, based on 1 article reviews. . No accessories are needed and SoftMax® Pro. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Enzyme Activation Analysis;. Simplified workflow: Add cellular imaging assays in a small footprint. SoftMax Pro User’s Manual v Contents Figures. User-upgradeable detection modules allow you to expand the capabilities of your microp. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Microplate Readers SpectraMax. If no sound is heard during initialization, contact technical support. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. Description 1. Molecular Interactions. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. GloMax Discover System. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. pH-Xtra Glycolysis Assay - Agilent. The chamber. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Prepare your iD series instrument for shipment. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. . This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Register to download your free eBook today. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. ImageXpress Confocal HT. . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1 Microplate Data Acquisition & Analysis Software for Windows. Multimode Reader Comparison Chart. . Our absorbance plate readers feature our PathCheck Sensor. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. SpectraMax i3x, onboard luminescence 121. Genscript cPass SARS-CoV-2 Neutralization Antibody Detection. VAL014, right table) assays on the SpectraMax i3x reader are shown below. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 58% for the detection module and 2. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 1. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 1. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Read height should be optimized for . A: percent GFP-positive cells calculated using the classification feature in the software. Analysis. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. com. Calcium. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. It allows you to explore cellular . Eliminate time-dependent substrate addition steps. The SpectraMax® i3x reader allows you to. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. The SoftMax® Touch Software is installed in. gen) on a Spectramax i3X luminometer. . Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. to View Figures. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Nucleic Acid and Protein Quantitation. 1. 2. . Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax® Microplate Readers and SoftMax® Pro Software. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Bioz Stars score: 98/100, based on 1 PubMed citations. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 3 Figures from 612 Publications Promega. The calculated LOQ values were 0. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Methods. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. spectramax i3x - by Bioz Stars , 2023-11. For wavelengths in the UV range above 220 nm,. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Also refer to this Multimode Reader Comparison Chart. Figure 3. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. However, no piece of equipment is immune to breaking down. i3x Specifications. ü; ü. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . ImageXpress Confocal HT. Description 1. to View Figures. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Quantiferon-TB Gold in Tube Kit. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Spectramax I3x, supplied by Danaher Inc, used in various techniques. 003 fmol 0. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. Prepare your i3/i3x instrument for shipment. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 314 low lamp level warning. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax i3x Register or Sign In. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Microplate readers, multi-mode, SpectraMax® i3x. 99. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Prepare your i3/i3x instrument for shipment. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Example: [Setup]. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. We would like to show you a description here but the site won’t allow us. . The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. As. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. infoboxeu@moldev. The calculated LOQ values were 0. Whether you are looking for an intuitive, easy-to-use reader for. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. +44-118-944-8000. Explore a wealth of applications with the future-ready SpectraMax i3x. Seller Details. 7 Figures from 1126 Publications. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. It allows you to explore cellular pathways and protein activation and expression in one system. Introduction. 5. . In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. GM2000 Register or Sign In. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. GFP expression imaged on the cytometer. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. D 1 1. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. SpectraMax i3x Multi-Mode Microplate Reader. com. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. Background Parallax,Columns 2. SoftMax Pro. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax i3x Multi-Mode Detection Platform. The information below applies to a standard 96 well plate, set to a linear shake mode. pathways and protein activation and expression in one system. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Bioz Stars score: 98/100, based on 1 PubMed citations. With optimized reagents, option to operate the readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Multimode Reader Comparison Chart. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. GM3000 Register or Sign In. g. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The calculated LOQ values were 0. See Unpacking the SpectraMax Paradigm Instrument on page 1. 315 can't find zero order. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Molecular Devices ScanLater Western Blot Detection Platform. Capture Flash Assays with Ease. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. . A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 1. Danaher Inc spectramax i3x. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Description 1. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 6% for the on-board optics (monochromators). These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. . Molecular Devices Software. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. 7. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. RIDASCREEN Gliadin competitive ELISA. Description. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. AlphaScreen. 2 or higher,. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Negative samples contained only MycoAlert Assay Buffer. . MC38 in vivo tumor model in hPD-1. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Find regional support. Not compatible with Home Edition. SpectraMax® Microplate Readers and SoftMax® Pro Software. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. Assay setup for a 384-well low-volume plate. The treatments were. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. 6% for the on-board optics (monochromators). ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 2 nM was calculated using SoftMax Pro Software. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. See SpectraMax® Microplate Reader User Manual Download Page. Controls containing all reaction. 313 reference gain check fail. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. SpectraMax Microplate Readers. 2 only) SpectraMax M-series Microplate readers. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. Image analysis provides accurate data. compound concentration, and an IC 50 value of 21. 12911151) 0. no. The SpectraMax i2X is used for cell migration, cell. Determination of anti-inflammatory. +44 118 944 8000. Curcumin binding assay. ü; SpectraMax® Mini reader support. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Find regional support. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. *On the SpectraMax i3x reader only. Bioz Stars score: 98/100, based on 1 PubMed citations. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Products : SoftMax Pro GxP Software, SoftMax Pro Software. We would like to show you a description here but the site won’t allow us. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Figure 1. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Overview of Western Blot Cartridge. GloMax ® Navigator System. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. . SpectraMax® ABS and ABS Plus reader support; ü. Molecular Devicesの全商品を見る. 030 fmol. to View Figures. Explore a wealth of applications with the future-ready SpectraMax i3x. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. 0.